The singer was recently on a press tour to promote a Netflix series.
Dolly Parton appeared on Late Night with Seth Meyers this past week and gave a special shout out to her gay fans through one of her most famous songs.
The 73-year-old singer was on the show to promote "Heartstrings," a Netflix original that uses her songs as the inspiration behind the episodes.
Meyers mentioned that his son loves the song "Jolene," so Dolly obliged the host by singing a few bars, only she changed a few of the lyrics for, as she puts it, her "gay crowd."
After she belted out the famous first verse of the country hit unchanged, she then dedicated the second part to the community with a special tweak.
“Drag queen, drag queen, drag queen, drag queen… please don’t take him just because you can.”
Although Parton has stayed silent politically, she has often acknowledged her LGBT fans and supported them publically.
"Hey, I think love is love and we have no control over that … I think people should be allowed to [marry]," she said about marriage equality back in 2017.